Coding

Part:BBa_M36926:Design

Designed by: Alexandra Ritchie, Scott Platshon   Group: Stanford BIOE44 - S11   (2013-06-09)


Beta-glucosidase A1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 12
    Illegal AgeI site found at 919
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 512


Design Notes

Changes to the sequence were made to optimize C-G content and codons, and minimize repeats and motifs for expression in E. coli

Source

This sequence came from Shewanella frigidimarina, a marine bacteria.

References